Rate of glucose transformation in the rat by whole-body analysis after blucose-14-C.

SHIPLEY, REGINALD A., ETHEL BUCHWALD CHUDZIK, AUSTIN P. GIBBONS, KATHLEEN JONGEDYK, AND DEWEY 0. BRUMMOND. Hate of glucose tmnsformation in the rat by whole-body analysis after glucose-W. Am. J. Physiol. 213(5): 1149-l 158. 1967.-Rate of disposal of glucose in the normal fasted rat, as calculated by Stewart-Hamilton dilution principle after a single injection of glucose-L-W, was 0.22 mg glucose carbon/min per 100 g rat. Analysis of the homogenized whole animal showed that rate of conversion to CO2 was 0.13 mg/min (61 y0 of total disposal), glvcogen 0.017, protein 0.029, lipid 0.011, and urine 0.029. The percentage of CO:! derived from glucose was 10%. Activity was recovered in a variety of subfractions of lipid. Urinary activity was chiefly in unidentified acidic compounds. Although multicompartmental analysis was not attempted, the behavior of several pools was characterized. Tracer glucose equilibrated so promptly with total body glucose that a single pool would be appropriate in a kinetic model. Composite moieties of acidic and basic compounds received 14C very rapidly; however, neither class of compounds behaved as a single pool. Specific activity curves for serum, muscle, and brain suggested that estracellular and intracellular bicarbonate compartments for the body as a whole would not be appropriate in an over-all model.